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Delphi-X Inverso Fluorescence – Microscope for University, Life Science and Industry

Delphi-X Inverso fluorescenceThe Delphi-X Inverso™ for fluorescence is an inverted microscope for the professional who needs to observe tissues or cells stained with fluorescence dyes.

Super contrast semi-apochromatic Fluarex™ objectives with collar adjustment ring assure perfect observation of cells or tissues in 1.2 mm thick Petri dishes or flasks.

Delphi-X Inverso fluorescence

The Delphi-X Inverso™ for fluorescence is an inverted microscope for the professional who needs to observe tissues or cells stained with fluorescence dyes.

Super contrast semi-apochromatic Fluarex™ objectives with collar adjustment ring assure perfect observation of cells or tissues in 1.2 mm thick Petri dishes or flasks.

Highlight of Microscope Delphi-X Inverso Fluorescence

  • Life science applications
  • Observation of fluorescence in tissues and cells
  • Brightfield, phase contrast fluorescence observations
  • DIC (differential interference contrast)
  • Long working distance IOS plan semi-apochromatic Fluarex achromatic objectives
  • Revolving sextuple nosepiece
  • 5 W LED or 100 W halogen transmitted illumination
  • 100 W HBO mercury vapor fluorescence illumination
  • Backwards tilting condenser arm
  • Easy placement of large objects
Delphi-X Inverso fluorescence microscope

Features

Delphi-X Inverso fluorescence feature

Models of Microscope Delphi-X Inverso Fluorescence

Model Objectives Illumination 100 W HBO fluorescence illumination
DI.3053‑PLPHFi
10x/20x/40x PLPHFi
5 W LED
Yes
DI.3053‑PLPHFi/HAL
10x/20x/40x PLPHFi
100 W Halogen
Yes

Product Specification​

EYEPIECE(S)

Pair of DIN HWF plan 10x/25 mm or 10x/22 mm eyepieces with diopter adjustment.

HEAD

Trinocular 45° inclined tubes. Interpupillary distance of 47 to 78 mm. Trinocular head with 100-0, 80/20 and 0/100 positions.

NOSEPIECE

Revolving sextuple nosepiece on ball-bearings. The nosepiece can be equipped with DIC prisms for the 10x/20x and 40x objectives.

OBJECTIVES

High numerical infinity semi-apochromatic long working distance plan phase Fluarex PLPHFi 10x/0.30, 20x/0.45, S40x/0.60 IOS objectives enable the sub-cellular structures. An optional 60x/0.80 IOS objective is also available.

The 20x, S40x and optional S60 objectives are equipped with collar adjustment ring for glass thicknesses from 0 to 1.2 mm.

Fluarex™ objectives are made of low-absorption glass. Fluarex™ is a trademark of Euromex microscopen bv. All optics are anti-fungus treated and anti-reflection coated for maximum light throughput.

STAGE

Stage 340 x 230 mm, equipped with a coaxial mechanical 130 x 85 mm X-Y stage. Supplied with:

  • Metal insert for O 38 mm cell culture dish
  • Metal insert for O 54 mm cell culture dish or 76 x 26 mm slides
  • Metal insert for Multiwell plates (127.76 x 85.48 mm)
  • Metal insert for Teraski plates (83.3 x 58.0 mm)
  • Optional universal cell culture holder

CONDENSER

Rotating N.A. 0.55 condenser with iris diaphragm. Working distance 26 mm. Supplied with phase contrast annuli for 10x/20x, 40x and 60x objectives and the DIC prisms for the 10x/20x/40x and 60x objectives. Three filter holders allow the insertion of Ø 38 mm filters in the optical path.

The arm with condenser can be tilted backwards for convenient access to the stage. This allows easy placement of large objects like flasks and Petri dishes.

LENS CHANGER

A lens changer with a 1.5x lens allow intermediary magnifications of 1.5 times the standard objective magnifications. Switching from 20x to 30x or 40 to 60 and from 60x to 90x magnification can be done without the need to re-focus on the sample.

Delphi-X_Inverso_Detail
6-Position fluorescence filter set wheel and 1.5 lens changer

FLUORESCENCE ATTACHMENT

The Delphi-X Inverso for fluorescence microscopy is supplied with a 6-position fluorescence filter set wheel and comes with two fluorescence filter sets for blue and green excitation (DI.9745 and DI.9746). Other filter sets are optional.

DI.9745 grafiek
DI.9745 Blue excitation, EX 460-495 DM 505 EM 510 long pass
DI.9746 Green excitation, EX 510-550 DM 570 EM 575 long pass
DX.9745 grafiek
DI.9747 Violet excitation, EX 400-410 DM 455 EM 460 Long pass
DX.9745 grafiek
DI.9748 Ultra violet excitation, EX 330-385 DM 410 EM 420 Long pass
DI.9750.TRITC Green excitation, EX 528-553 DM 565 EM 578-633 band pass
DX.9745 grafiek
DI.9750.FITC Blue excitation, EX 460-495 DM 505 EM 510-550 band pass
DX.9745 grafiek
DI.9750.DAPI UV excitation, EX 360-390 DM 415 EM 435-485 band pass
DX.9745 grafiek
DI.9750.R Red excitation, EX 620-650 DM 660 EM 670-750 band pass

FOCUSING ADJUSTMENT

Coaxial 10 mm coarse and fine adjustments, 1 μm precision and with friction adjustment.

ILLUMINATION

Fluorescence illumination with a 100 W mercury vapor lamp with external 100-240 V power supply. Transmitted 5 W LED or 100 W halogen illumination and internal power supply. Fuse holder 3.15 A / 250 V.

PACKAGE CONTENT

Supplied with metal insert for Ø 38 mm cell culture dish and a metal insert for Ø 54 mm cell culture dish with 76 x 26 mm slides, metal insert for Multiwell plates, metal insert for Teraski plates, blue and green interference Ø 45 mm filters, power cord, dust cover, green filter and alignment telescope and user manual.

Accessories

EYEPIECES

DI.6010

Super wide field SWF 10x/25 mm focusable eyepiece for Delphi-X Inverso, 30 mm diameter tube

DI.6210

Super wide field SWF 10x/22 mm focusable eyepiece for Delphi-X Inverso, 30 mm diameter tube

DI.6012

Wide field WF 12.5x/17.5 mm eyepiece

DI.6015

Wide field WF 15x/16 mm eyepiece

DI.6020

Wide field WF 20x/12 mm eyepiece

DI.6099-L

Eyecups for eyepiece

OBJECTIVES

DI.8704

Infinity Plan Semi-Apochromatic Phase contrast Fluarex PLPHFi-Apo 4x/0.13 objective, working distance 16,8 mm. Cover glass correction 1.2 mm. Needs phase annuli for 4x objective DI.8404

DI.8710

Infinity semi-apochromatic long working distance plan phase Fluarex PLPHFi 10x/0.30, IOS objective PLPHi 10x/0.30 objective. WD 10.2 mm

DI.8720

Infinity semi-apochromatic long working distance plan phase Fluarex PLPHFi 20x/0.45 objective. WD 7.5 to 8.8 mm

STANDARD GENERAL PURPOSE FLUORESCENCE FILTER SETS

Long pass type:
DI.9745-6

Standard filter set for Blue excitation EX 460-495 nm (BP) DM 505 EM 510 nm (LP). Needs empty cube

DI.9746-6

Standard filter set for green excitation EX 510-550 nm (BP) DM 570 EM 575 (LP) Needs empty cube

DI.9747-6

Standard filter set for Violet excitation EX 400-410 nm (BP) DM 455 EM 460 (LP) Needs empty cube

DI.9748-6

Standard filter set for UV excitation EX 330-385 nm (BP) DM 410 EM 420 (LP) Needs empty cube

DI.9749

Empty cube for Delphi-X Inverso Fluorescence attachment

Band-pass type :
DI.9750.19000

Fluorescence filter set EX375 (BP28: 361-389 nm) DM415 (LP) EM435 (LP) optimized for UV/DAPI, Calcofluor White. Needs empty filter block

DI.9750.19006

Fluorescence filter set EX560 (BP40: 540-580 nm) DM600 (LP) EM610 (LP) optimized for FM 4-64, Turbo FP650. Needs empty filter block

DI.9750.19008

Fluorescence filter set EX450 (BP50: 425-475 nm) DM485 (LP) EM495 (LP) optimized for Auramine Longpass. Needs empty filter block

DI.9750.19010

Fluorescence filter set EX480 (BP30: 465-495 nm) DM505 (LP) EM600 (LP) optimized for FM 1-43 / 4-64 , Pulsar 650 and Chlorophyll Longpass. Needs empty filter block

DI.9750.19011

Fluorescence filter set EX405 (BP30: 390-420 nm) DM505 (LP) EM450 (LP) optimized for Coumarin and Pacific Blue Longpass. Needs empty filter block

DI.9750.19012

Fluorescence filter set EX375 (BP28: 361-389 nm) DM415 (LP) EM515 (LP) optimized for FluoroGold and CalcoFluor White Longpass. Needs empty filter block

DI.9750.39000

Fluorescence filter set EX375 (BP28: 361-389 nm) DM415 (LP) EM460 (BP50: 435-485 nm) optimized for DAPI, Hoechst, AlexaFluor 350. Needs empty filter block

DI.9750.39001

Fluorescence filter set EX435 (BP20: 425-445 nm) DM455 (LP) EM480 (BP30: 465-495 nm) optimized for AmCyan, Atto 425, Cerulean, CFP, ECFP fluorophore. Needs empty filter block IS.9749

DI.9750.39002

Fluorescence filter set EX480 (BP30: 465-495 nm) DM505(LP) EM535 (BP40: 515-555 nm) optimized for Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium, Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald, GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block

DI.9750.39003

Fluorescence filter set EX495 (BP20: 485-505 nm) DM515 (LP) EM540 (BP30: 525-555 nm) optimized for Alexa Fluor 514™, Citrine, EYFP/pH 7, F/lAsH-CCPFCC, mCitrine, Oregon Green™ 514, Rhodamine 123, Topaz. Needs empty filter block

DI.9750.39004

Fluorescence filter set EX540 (BP25: 528-553 nm) DM565 (LP) EM605 (BP55: 578-633 nm) optimized for Alexa Fluor 546™, Alexa Fluor 555™, Atto 550, CAL, Fluor® Red 590, Cy3™, DiI, DsRed, DyLight 549, MitoTracker Orange/MeOH, Resorufin, Rhod-2, TagRFP, TAMRA tdTomato, Tetramethylrhodamine, isothiocyanate, TRITC. Needs empty filter block

DI.9750.39005

Fluorescence filter set EX540 (BP25: 528-552 nm) DM565 (LP) EM 620 (BP60: 590-650 nm) optimized for TRITC-red-shifted, Ethidium homidimer-1/DNA, Propidium Iodide. Needs empty filter block

DI.9750.39007

Fluorescence filter set EX620 (BP50: 595-645 nm) DM655 (LP) EM 690 (BP50: 665-715 nm) optimized for Alexa Fluor 647™, Allophycocyanin (APC), Atto 647N, Cy5™, DiD, Draq5, DyLight 649, MitoTracker Deep Red 633/MeOH, Nile Blue, Quasar® 670, SYTO® 60, TO-PRO™-3. Needs empty filter block

DI.9750.39008

Fluorescence filter set EX420 (BP40: 400-440) DM455 (LP) EM 535 (BP40: 515-555) optimized for Lucifer Yellow/Tetracycline/AlexaFluor 430. Needs empty filter block

DI.9750.39009

Fluorescence filter set EX480 (BP30: 465-495) DM505 (LP) EM 620 (BP60: 590-650) for Acridine Orange + RNA, Di-8-ANEPPS, non-radiometric, DiA, Ethidium Bromide, FM™ 1-43, LysoTracker Yellow HCK-123, Pulsar™ 650. Needs empty filter block

DI.9750.39010

Fluorescence filter set EX560 (BP40: 540-580) DM600 (LP) EM635 (BP60: 615-665) optimized for Texas Red, mCherry, AlexaFuor 594. Needs empty filter block IS.9749

DI.9750.49000

Fluorescence filter set EX350 (BP50: 325-375) DM400 (LP) EM460 (BP50: 435-485) optimized for Texas Red, mCherry, AlexaFuor 594. Needs empty filter block IS.9749

CAMERA ACCESSORIES

DI.9810

C-mount with 1x magnification for C-mount camera

DI.9840

C-mount with high resolution 0.40x objective for 1/3 inch C-mount camera

DI.9850

C-mount with high resolution 0.50x objective for 1/2 inch C-mount camera

DISPOSABLES

PB.5155

Microscope slides 76 x 26 mm, ground edges, 50 pieces per pack

PB.5157-W

Microscope slides 76 x 26 mm, ground edges. White frosted side, 50 pieces per pack

PB.5157-B

Microscope slides 76 x 26 mm, ground edges. Blue frosted side, 50 pieces per pack

PB.5160

Microscope slides 76 x 26 mm with cavity depth 0.25 mm, grinded edges. 10 Pieces per pack

PB.5165

Cover glasses 18 x 18 mm, 0.13-0.17 mm, 100 pieces

PB.5168

Cover glasses 22 x 22 mm, 0.13-0.17 mm. 100 pieces

PB.5245

Lens cleaning paper, 100 sheets per pack

PB.5274

Iso propyl alcohol 99%, 200 ml

PB.5275

Cleaning kit: lens fluid, lint free lens tissue paper, brush, air blower, cotton swabs

MISCELLANEOUS

AE.5214

Fuses 500 mA 250 V, per 10 pcs. models with LED

AE.5216

Fuses 5A 250 V, per 10 pcs. models with 100 W halogen

More Information

Get more information and English brochure for this Microscope for University, Life Science and Industry here!

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